Investigating inflammatory processes of plaque destabilization using proteomics
SSAR 2022
Lasse Lorentzen
Extracellular matrix remodeling in atherosclerosis
Extracellular matrix remodeling in atherosclerosis
Remodeling of the vascular extracellular matrix (ECM) takes place in all stages of disease
Both synthesis and degradation of ECM proteins is altered in atherosclerosis
Hypothesis:
Remodeling of the extracellular matrix (ECM) is a key driver in plaque destabilization
Proteomics investigation of ECM remodelling
Proteomics workflow
Study design
21 symptomatic plaques from the carotid artery
7 Hard, 7 Mixed, 7 Soft plaques
Compared by proteomics
Differentially regulated proteins
Over-representation analysis
N-terminomics investigation of protelytic ECM remodelling
ECM degradation by proteolytic enzymes
ECM degradation by proteolytic enzymes
ECM degradation by proteolytic enzymes
Proteomics workflow